Description
The Real-time PCR System YR06248 / YR06249 combines cutting-edge signal collection and spectrum separation technology with a unique temperature control mechanism, achieving an exceptional level of detection sensitivity, multi-color crosstalk correction, temperature uniformity, and accuracy. Available in both 4-channel and 5-channel configurations, this system is versatile in accommodating a wide range of qPCR dye-based and probe-based chemistries, facilitating multiplex PCR applications with ease. In addition, it supports various standard analysis methods such as absolute quantification, genotype analysis, HRM, MMCA, and gene expression analysis.
Market Price
The market price for advanced PCR systems like the YR06248 / YR06249 typically ranges between $22,500 and $23,760 USD. This competitive pricing reflects the high-quality and robust performance of the system, making it an attractive choice for laboratories seeking reliable and efficient real-time PCR solutions.
Frequently Asked Questions
- Is the system compatible with all types of qPCR reagents? Yes, the system is compatible with a broad spectrum of qPCR dye-based and probe-based chemistries.
- What is the sample capacity of this PCR system? The system supports an 8×0.1 mL PCR strip x12, 96×0.1 mL individual tubes, and a 0.1 mL PCR Plate.
- Can this system perform high-resolution melting (HRM) analysis? Yes, it supports HRM and other advanced analysis techniques.
Advantages and Disadvantages
Advantages: The system offers fast and precise thermal cycling, excellent reproducibility, and high resolution with a dynamic range of up to 10 orders of magnitude.
Disadvantages: The complexity of features may require comprehensive training for unexperienced users to fully utilize its potential.
Product Use in the Field
This real-time PCR system is essential in various fields such as clinical diagnostics, biotechnology, and research laboratories. It enables researchers and practitioners to conduct accurate gene quantification, genotyping studies and analyze gene expression, thereby playing a crucial role in advancing genomic research and medical diagnostics.
Recommendations
To maximize the lifespan and performance of the Real-time PCR System YR06248 / YR06249, it is recommended to regularly calibrate and maintain the equipment according to the manufacturer’s guidelines. Additionally, utilizing high-quality reagents and ensuring proper training for users can significantly enhance the results and efficiency of your experiments.
Features
- Fast and Precise Thermal Cycling
- Innovative Optic Design
- Excellent Reproducibility
- Up to 10-log Dynamic Range
- Melting Curve Analysis
- High Resolution
Technical Specifications
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
